Content
- Lectures: 34 hours
- Seminar: 20 hours
- Exercises 12 hours
- Independent work: 114 hours
Subject carrier
Description
General information on the cell and methods in cell biology
• the development of a cell and evolution
• light and electron microscopy
• Prokariont, eukariont cell
• model organisms ·
Basic components – macromolecules
• overview
• lipids and their functions, cell membranes
• protein functions – cytoskeleton, antiobodies, motor proteins
Cell structure and function of the organeles
• Nucleus
• Mitochondria
• Endoplasmic reticulum, Golgi aparatus
DNA and DNA related processes
• Structure, chromosomes, genes
• Replication
• transcription, translation, posttranslational modifications
• sorting of proteins within the cell, nuclear pores, vesicular transport
Mutations
• DNA damage, repair, recombination
• Causes and consequences of mutations
Cell cycle
• Cell cycle phases
• Mitosis
• Cell cycle regulation
• Meiosis
• Basis of genetics and inheritance
Cell death
• Apoptosis,
• necrosis ·
Cell communication
• receptors, signal molecules
Specific types of cells
• Stem cells
• Tumor cells
• Blood cells and bodies
Practicals:
• Safety at work and laboratory work order
• Cell morphology under invert microscope
• Cell counting, use of hemocytometer
• Cell survival test
• DNA isolation from biological material
