Ime in priimek: assoc. prof. Zala Jenko Pražnikar, PhD
Links: ARIS 24263, 
E-Mail: zala.praznikar@upr.si

Research facility:
University of Primorska, Faculty of Health Sciences
Institut Jožef Stefan

Research field according to ARIS classification
Science: Medical sciences
Field: Metabolic and hormonal disorders / Public health

Summary of research topic and field
Metals, although accounting for only 2.5% of the human body’s mass, are crucial for numerous physiological processes. Their distribution is precisely regulated under normal physiological conditions; however, diseases can disrupt this balance, leading to altered biological functions. Recent advances in analytical techniques have highlighted the potential of metal isotopes as novel biomarkers for early disease diagnosis. Isotopic metallomics leverages natural variations in isotope ratios, which change due to pathological processes, enabling sensitive and specific diagnostic indicators. This project will focus on characterizing the isotopic composition of specific essential elements in serum, urine, and feces in both healthy individuals and those with prediabetes. In the proposed project, we aim to establish baseline values of isotopic variability in healthy individuals while considering factors such as gender, diet, and lifestyle. By comparing these baseline values with data from individuals with prediabetes, we will identify specific isotopic deviations. Additionally, the project will explore interactions between isotopes and other “omics,” such as the microbiome and metabolome, to deepen our understanding of metabolic dysfunctions. The main goal of the project is to identify early irregularities in metal homeostasis and metabolic dysregulation, which will, in the coming years, contribute to the development of diagnostic tools based on isotope ratios. This approach has the potential to shift towards preventive and personalized medicine and help address the global burden of metabolic diseases.